Publications

Original papers

2022

1. The long nanowire DNA stamper transfers gene directly into brain cells ex vivo and in vivo.
   Numano R, Goryu A, Kubota Y, Sawahata H, Yamagiwa S, Matsuo M, Iimura T, Tei H, Ishida M and Kawano T
   FEBS Open Bio. 2022 Apr; 12(4): 835–851.
2. Influence of Electroporation Medium on Delivery of Cell-Impermeable Small Molecules by Electrical Short-Circuiting via an Aqueous Droplet in Dielectric Oil: A Comparison of Different Fluorescent Tracers.         
   Watanabe Y, Nihonyanagi H, Numano R, Shibata T, Takashima K, Kurita
   Sensors (Basel). 2022 Mar 24;22(7):2494.
3.  A floating 5 μm-diameter needle electrode on the tissue for damage-reduced chronic neuronal recording in mice.
   Yamashita K, Sawahata H, Yamagiwa S, Yokoyama S, Numano R, Koida K,  Kawano T.
   Lab on a Chip, Vol. 22, No. 4, pp. 747-756, 2022.
4. Nanoneedle-Electrode Devices for In Vivo Recording of Extracellular Action Potentials.
   Banno T, Tsuruhara S, Seikoba Y, Tonai R, Yamashita K, Idogawa S, Kita Y, Suzuki K, Yagi Y, Kondo Y, Numano R, Koida K, Kawano T.
   ACS Nano. 16(7):10692-10700 ,2022 Jul.
5.  Influence of Electroporation Medium on Delivery of Cell-Impermeable Small Molecules by Electrical Short-Circuiting via an Aqueous Droplet in Dielectric Oil: A Comparison of Different Fluorescent Tracers.
   Watanabe Y, Nihonyanagi H, Numano R, Shibata T, Takashima K, Kurita H.
   Sensors (Basel). 22(7):2494. 2022 Mar.
6. Nanoscale-tipped wire array injections transfer DNA directly into brain cells ex vivo and in vivo.
   Numano R, Goryu A, Kubota Y, Sawahata H, Yamagiwa S, Matsuo M, Iimura T, Tei H, Ishida M, Kawano T.
   FEBS Open Bio.12(4):835-851. 2022 Apr.

2121

1. Three-micrometer-diameter needle electrode with an amplifier for extracellular in vivo recordings.
   Kita Y, Tsuruhara S, Kubo H, Yamashita K, Seikoba Y, Idogawa S, Sawahata H, Yamagiwa S, Leong XLA, Numano R, Koida K, Kawano T.
   Proc Natl Acad Sci U S A.2021 Apr 20;118(16)
   

2020

1. Mechanistic studies of gene delivery into mammalian cells by electrical short-circuiting via an aqueous droplet in dielectric oil.
   Kurita H, Nihonyanagi H, Watanabe Y, Sugano K, Shinozaki R, Kishikawa K, Numano R, Takashima K. PLoS One. 2020 Dec 4;15(12):e0243361. doi: 10.1371/journal.pone.0243361. eCollection 2020.
2. Introduction of a plasmid and a protein into bovine and swine cells by water-in-oil droplet electroporation.                         　　　　　　　　　　　　　　　　　　　　　　　　　　　　Ishino T, Kurita H, Kirisawa R, Shimamoto Y, Numano R, Kitamura H
   J. Vet. Med. Sci., 82(1),p14–22

2019

1. Overcoming a major obstacle to the practical application of regenerative medicine: Paving the way for the mass production of iPS Cells.
   Numano R
   TUT Research No.17, 2019年5月
2. A Magnetically Assembled High-Aspect-Ratio Needle Electrode for Recording Neuronal Activity.
   Yasui T, Yamagiwa S, Sawahata H, Idogawa S, Kubota Y, Kita Y, Yamashita K, Numano R, Koida K, Kawano T.
   Adv Healthc Mater.e1801081., 2019
3. Donut-Shaped Stretchable Kirigami: Enabling Electronics to Integrate with the Deformable Muscle.
   Morikawa Y, Yamagiwa S, Sawahata H, Numano R, Koida K, Kawano T.
   Adv Healthc Mater. 2019 Nov 7:e1900939. doi: 10.1002/adhm.201900939. , 2019
4. Flexible parylene-thread bioprobe and the sewing method for in vivo neuronal recordings
   Yamashita K, Sawahata H, Yamagiwa S, Morikawa Y, Numano R, Koida K, Kawano T
   Sensors and Actuators B: Chemical 316,p127835-127835, 2019
5. Introduction of a plasmid and a protein into bovine and swine cells by water-in-oil droplet electroporation.
   Ishino T, Kurita H, Kirisawa R, Shimamoto Y, Numano R, Kitamura H
   The Journal of veterinary medical science 82(1),p14-22,
6. Flow cytometric study on mechanism of gene delivery into mammalian cells by electrostatic actuation of a water droplet in dielectric oil                 　　　　　　　　　　　　　　　　　　　Kurita H, Sugano K, Kishikawa K, Numano R, Takashima K　　　　　　　　　　　　　　　　　　3rd World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine, and Food & Environmental Technologies
7. Experimental and Computational Study on Efficient Intracellular Delivery Through Microdroplet-Based Electroporation　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　Ishii-Teshima M, Kurita H, Numano R, Nagai M, Shibata T　　　　　　　　　　　　　　　　　　　The 8th International Conference of Asian Society for Precision Engineering and Nanotechnology (ASPEN 2019)
8. Three Dimensional Core-Shell Microneedle-Electrode for Multisite Neuronal Recoding　　　　Idogawa S, Kubota Y, Yamagiwa S, Sawahata H, Numano R, Koida K, Kawano T　　　　　　　　　20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII)
9. A High-Density Array of 3D Microneedle-Electrodes for Evaluation of Spatial Resolution of Neuronal Activity　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　Kotani Y, Sawahata H, Yamagiwa S, Numano R, Koida K, Kawano T　　　　　　　　　　　　　　20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII)
10. Floating 5-μm-Diameter Needle for Low Invasive Chronic Recording　　　　　　　　　　　Yamashita K, Sawahata H, Yamagiwa S, Numano R, Koida K, Kawano T　　　　　　　　　　　　20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII)
11. Sewing Bioprobe　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　Yamashita K, Sawahata H, Yamagiwa S, Morikawa Y, Numano R, Koida K, Kawano T　　　　　　　IEEE 32nd International Conference on Micro Electro Mechanical Systems (MEMS) ,p621-624

2018

1. 幹細胞研究の最前線〜基礎から臨床まで〜 液滴電気穿孔装置の開発と幹細胞研究への応用
   沼野　利佳, 篠崎 竜登, 松尾 美奈子, 栗田 弘史, 松本 光二朗, 赤松 和士
   Cytometry Research 28(Suppl.) 29-29 2018年5月
2. A central-acting connexin inhibitor, INI-0602, prevents high-fat diet-induced feeding pattern disturbances and obesity in mice.
   Sasaki T, Numano R, Yokota-Hashimoto H, Matsui S, Kimura N, Takeuchi H, Kitamura T.
   Mol Brain, 11,p11-28, 2018
3. Ultrastretchable Kirigami Bioprobes. 
   Morikawa Y, Yamagiwa S, Sawahata H, Numano R, Koida K, Ishida M, Kawano T
   Adv Healthc Mater., 7(3) , 2018
4. Fiber bundle endomicroscopy with multi-illumination for three-dimensional reflectance image reconstruction     
   Ando Y, Sawahata H, Kawano T, Koida K, Numano R
   J Biomed Opt,3(2),p1-4. , 2018
5. Long nanoneedle-electrode devices for extracellular and intracellular recording in vivo.             
   Kubota Y, Yamagiwa S, Sawahata H, Idogawa S, Tsuruhara S, Numano R, Koida K, Ishida M, Kawano T
   Sensors and Actuators B: Chemical, 258, p1287-1294, 2018
6. Development of microdroplet-based electroporation system for gene transfection　　　　　　　Ishii-Teshima M, Ushiroda F, Nagai M, Numano R, Shibata T　　　　　　　　　　　　　　　　　The 44th International Conference on Micro and Nano Engineering 2018 (MNE 2018)
7. Long nanowire array for in vivo in vitro DNA injections into cells in brain tissues   　　　　　　Kubota Y, Numano R, Goryu A, Sawahata H, Yamagiwa S, Matsuo M, Ishida M, Kawano T　　　　　The 31th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2018)
8. Dissolvable material-sheathed microneedle-electrode device slid into a narrow gap of the brain　Yamagiwa S, Sawahata H, Numano R, Ishida M, Koida K, Kawano T　　　　　　　　　　　　　　The 31th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2018)
9. Stretchable micro-doughnuts Kirigami Bioporobe         　　　　　　　　　　　　　　　　Morikawa Y, Yamagiwa S, Sawahata H, Numano R, Koida K, Ishida M, Kawano T　　　　　　　　　The 31th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2018)
10. Single needle electrode–topped amplifier package (STACK) for in vivo applications　　　　　　　Kita Y, Kubo H, Sawahata H, Yamagiwa S, X.L.A. Leong, Numano R, Koida K, Ishida M, Kawano T 　The 31th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2018)

2017

1. Bone Resorption Is Regulated by Circadian Clock in Osteoblasts.
   Takarada T, Xu C, Ochi H, Nakazato R, Yamada D, Nakamura S, Kodama A, Shimba S, Mieda M, Fukasawa K, Ozaki K, Iezaki T, Fujikawa K, Yoneda Y, Numano R, Hida A, Tei H, Takeda S, Hinoi EJ,
   Journal Of Bone And Mineral Research,32, p872-881, 2017
2. The intrinsic microglial clock system regulates interleukin-6 expression.
   Nakazato R, Hotta S, Yamada D, Kou M, Nakamura S, Takahata Y, Tei H, Numano R, Hida A, Shimba S, Mieda M, Hinoi E, Yoneda Y, Takarada T.
   Glia 2017,65(1):198-208
3. Ultra high-aspect-ratio neuroprobe: 5-μm-diameter and 400-μm-length needle detects action potentials in VIVO　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　Yamagiwa S, Sawahata H, Oi H, Numano R, Ishida M, Koida K, Kawano T　　　　　　　　　　　　IEEE 30th International Conference on Micro Electro Mechanical Systems (MEMS)　,p553 – 556  
4. In vivo neuronal recordings using three-dimensional microneedle-electrode assembled on flexible substrate 　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　Yamagiwa S, Sawahata H, Numano R, Ishida M, Koida K, Kawano T　　　　　　　　　　　　　　IEEE 30th International Conference on Micro Electro Mechanical Systems (MEMS) ,p1722 – 1725
5. In  transfection methodology for mammalian cells using water-in-oil droplet deformation in a DC electric field　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　Kurita H, Kishikawa K, Takao Y, Numano R, Takashima K, Mizuno A　　　　　　　　　　　　　　The 2nd World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine, and Food & Environmental Technologies

2016

1. Fundamental study on agenetransfection methodology for mammalian cellsusingwater-in-oildropletdeformationinaDC electric field
   Kurita H, Takao;Y,, Kishikawa K, Takashima K, Numano R, Mizuno A.
   Biochemistry andBiophysicsReports,査読有,2016,8,p81–88
2. In vivo bioluminescence and reflectance imaging of multiple organs in bioluminescence reporter mice by bundled-fiber-coupled microscopy,
   Ando Y, Sakurai T, Koida K, Tei H, Hida A, Nakao K, Natsume M, Numano R
   Biomed.Opt.Express,査読有,2016,7(3),p963-978
3. Reflectance Imaging by Fiber Bundle Endoscope: Vertical Reconstruction by Multipositional Illumination
   Ando Y, Koida K, Sawahata H, Sakurai T, Natsume M, Kawano T, and Numano R,
   AIP proceedings,査読有,2016
4. Nanoscale-Tipped High-Aspect-Ratio Vertical Microneedle Electrodes for Intracellular Recordings
   Kubota Y, Oi H, Sawahata H, Goryu A, Ando Y, Numano R, Ishida M, Kawano T..
   Small,査読有,2016,12(21):2846-53
5. Intracellular Electrodes: Nanoscale-Tipped High-Aspect-Ratio Vertical Microneedle Electrodes for Intracellular Recordings
   Kubota Y,Oi H, Sawahata H,　Goryu A,Ando Y, Numano R, Ishida M, Kawano T
   Small,12(21), p2803–2931, 2016
6. Single 5 μm diameter needle electrode block modules for unit recordings in vivo.  
   Sawahata H, Yamagiwa S, Moriya A, Dong T, Oi H, Ando Y, Numano R, Ishida M, Koida K, Kawano T.
   Scientific Reports,2016,25;6:35806
7. 細胞への遺伝子導入の革新的新法
   「次世代型液滴エレクトロポレーション」の開発
   – iPS細胞の大量製造など医療応用にむけたものつくり技術 –
   沼野　利佳
   クリーンテクノロジー、日本工業出版 201６年１１月号　

2015

1. Novel parallelized electroporation by electrostatic manipulation of a water-in-oil droplet as a microreactor, e0144254 (15pp), 2015
   Kurita H, Takahashi S, Asada A, Matsuo M, Kishikawa K, Mizuno A, Numano R
   PLoS One,2015,10(12)
2. Dissolvable Base Scaffolds Allow Tissue Penetration of High-Aspect-Ratio Flexible Microneedles
   Yagi S, Yamagiwa S, Kubota Y, Sawahata H, Numano R, Imashioya T, Oi H, Ishida M, Kawano T.
   Adv Healthc Mater. ,2015,in press
3. Low glucose-induced ghrelin secretion is mediated by ATP-sensitive potassium channe
   Manami Oya1, Tetsuya Kitaguchi2,3, Kazuki Harada1, Rika Numano4,
   Takahiro Sato5, Masayasu Kojima5, Takashi Tsubo
   Journal of Endocrinology,2015,226(25-34).
4. Layer-by-layer assembled nanorough iridium-oxide/platinum-black for low-voltage microscale electrode neurostimulation
   Shota Yamagiwaa, Akifumi Fujishiroa, Hirohito Sawahataa, Rika Numanob,c,Makoto Ishidaa,b, Takeshi Kawanoa,^∗
   Sensors and Actuators B: Chemical ,2015,206(205–211)

2014

1. Short hairpin RNAs of designed sequences can be extracellularly produced by the marine bacterium Rhodovulum sulfidophilum
   Nagao N, Suzuki H, Numano R, Umekage S, Kikuchi Y
   J Gen Appl Microbiol. ,2014,60（222～ 226）
2. Melanopsin resets circadian rhythms in cells by inducing clock genePeriod
   Shuhei Yamashita, Tomoe Uehara, Minako Matsuo, Yo Kikuchi and Rika Numano
   AIP Conf. Proc. ,2014,1585, 40-44
3. A vertically integrated nanoscale tipped microprobe intracellular electrode arra
   Kubota, Y. ,Oi, H. , Sawahata, H. , Goryu, A., Ando, Y., Numano R, Ishida, M. , Kawano,
   Micro Electro Mechanical Systems (MEMS) ,2014,155 – 158

2013

1. Vesicular nucleotide transporter is involved in ATP storage of secretory lysosomes in astrocyte
   Oya M, Kitaguchib T, Yanagihara Y, Numano R, Kakeyama M, Ikematsu K, Tsuboi
   Biochem Biophys Res Commun ,2013,16(145～151）

2012

1. Small GTPase Cdc42 modulates the number of exocytosis-competent dense-core vesicles in PC12 cells.
   Sato M, Kitaguchi T, Numano R, Ikematsu K, Kakeyama M, Murata M, Sato K, Tsuboi T
   Biochem Biophys Res Commun ,2012,30(254-269）
2. Nanoscale tipped microwire arrays enhance electrical trap and depth injection of nanoparticles
   Goryu A, Numano R, Ikedo A, Ishida M, Kawano T
   Nanotechnology. ,2012,23（2741～ 2705）
3. A fluorescence spotlight on the clockwork development and metabolism of bone
   Tadahiro Iimura* , Ayako Nakane, Mayu Sugiyama, Hiroki Sato, Yuji Makino, Takashi Watanabe, Yuzo Takagi, Rika Numano*, Akira Yamaguchi
   J Bone Miner Metab ,2012,24（55～ 63）

2011

1. 骨の成長と代謝における概日リズムの働き －蛍光イメージングによるアプローチと展望－
   Ayako Nakane, Rika Numano* ,Yuzo Takagi , Akira Yamaguchi, Tadahiro Iimura
   Jounal of Bone morphometry,2011,21（41-50）
2. 双方向の光反応性を持つYin/Yang受容体
   細胞工学、羊土社 2011年8月号　平成23年
   沼野　利佳
3. Fluorescent imaging approach and perspective for the circadian development and metabolism of bone
   Jounal of Bone morphometry. Vol.21, p41-50, 2011
   Nakane A, Numano R*, Takagi Y, Yamaguchi A, Iimura T

2009

1. Nanosculpting reversed wavelength sensitivity into a photoswitchable iGluR.
   Numano R, Szobota S, Lau AY, Gorostiza P, Volgraf M, Roux B, Trauner D, Isacoff EY
   Proc Natl Acad Sci U S A,106, 2009,p6814-6819
2. Ontogeny of circadian organization in the rat.
   Yamazaki S, Yoshikawa T, Biscoe EW, Numano R, Gallaspy LM, Soulsby S, Papadimas E, Pezuk P, Doyle SE, Tei H, Sakaki Y, Block GD, Menaker M^＊.
   J Biol Rhythms., 24, 2009,　p55-63

2007

1. Remote control of neuronal activity with a light-gated glutamate receptor.
   Szobota S, Gorostiza P, Del Bene F, Wyart C, Fortin DL, Kolstad KD, Tulyathan O, Volgraf M, Numano R, Aaron HL, Scott EK, Kramer RH, Flannery J, Baier H, Trauner D, Isacoff EY.
   Neuron, 54, 2007, p535-545
2. Mechanisms of photoswitch conjugation and light activation of an ionotropic glutamate receptor.
   Gorostiza P, Volgraf M, Numano R, Szobota S, Trauner D, Isacoff EY.
   Proc Natl Acad Sci U S A, 104, 2007,p10865-10870
3. Remote control of neuronal activity with a light-gated glutamate receptor.
   Szobota S, Gorostiza P, Del Bene F, Wyart C, Fortin DL, Kolstad KD, Tulyathan O, Volgraf M, Numano R, Aaron HL, Scott EK, Kramer RH, Flannery J, Baier H, Trauner D, Isacoff EY＊.
   Neuron, 54, 2007, p535-545

2006

1. Constitutive expression of the Period1 gene impairs behavioral and molecular circadian rhythms.
   Numano R, Yamazaki S, Umeda N, Samura T, Sujino M, Takahashi R, Ueda M, Mori A, Yamada K, Sakaki Y, Inouye ST, Menaker M, Tei H.
   Proc Natl Acad Sci U S A, 103,p3716-3721, 2006
2. Allosteric control of an ionotropic glutamate receptor with an optical switch.
   Volgraf M, Gorostiza P, Numano R, Kramer RH, Isacoff EY, Trauner D.
   Nat Chem Biol., 2, p47-52, 2006

2004

1. Temporal precision in the mammalian circadian system: a reliable clock from less reliable neurons.
   Herzog ED, Aton SJ, Numano R, Sakaki Y, Tei H.
   J Biol Rhythms.,19,p35-46, 2004

2002

1. Transgenic rats in study of mammalian circadian rhythms.
   Numano R
   Anitex, 14, p203-209, 2002

2001

1. The coupling of central and peripheral circadian oscillators in the mammalian circadian system by means of Period1 expression profile
   Numano R, Tei H
   Shinkei Kenkyu no Shinpo, 45, p734-743, 2001
2. The coupling of central and peripheral circadian oscillators in the mammalian circadian system by means of Period1 expression profile
   Shinkei Kenkyu no Shinpo, 45巻（734頁～743頁）平成13年
   Numano R, Tei H

2000

1. Resetting central and peripheral circadian oscillators in transgenic rats.
   *Yamazaki S, *Numano R, *Abe M, Hida A, Takahashi R, Ueda M, Block GD, Sakaki Y, Menaker M, Tei H.
   Science, 査読有,288,p682-685, 2000　　*equally contributed

1998

1. 2,2′-Azobis (4-methoxy-2,4-dimethylvaleronitrile), a new lipid-soluble azo initiator: application to oxidations of lipids and low-density lipoprotein in solution and in aqueous dispersions.
   Noguchi N, Yamashita H, Gotoh N, Yamamoto Y, Numano R, Niki E.
   Free Radic Biol Med., 24, p259-268, 1998
2. Oxidation of lipids in low density lipoprotein particles.
   Noguchi N, Numano R, Kaneda H, Niki E.
   Free Radic Res., 29, p43-52, 1998
3. Identification of the mammalian homologues of the Drosophila timeless gene, Timeless1.
   Koike N, Hida A, Numano R, Hirose M, Sakaki Y, Tei H.
   FEBS Lett., 441,p427-431, 1998
4. Human clock controlled gene, Period1
   Numano R , Tei H , Sakaki Y
   Medical Technology, 26, p213-215, 1998